Once the facility is already built and we assume everything is mechanically going well, your goal is to keep the microbes happy. Unhappy microbes = reduced biogas production.
Common operational factors that stress out the microbes:
-Overfeeding
-Temperature swings (more common in the winter when feedstocks are fed frozen into warm digester)
-Nutrient deficiency for high fat digesters or digesters with limited feedstock variety
-Changing parameters (T, pH, feeding rate) faster than the microbes can react
-Inhibition or toxicity (there are many types of microbe inhibition)
I’ve linked an article about the Top 10 Things to Make Your Digester Biology Happy. It’s a starting point for understanding some of the biological operational factors that influence biogas production.
https://azuraassociates.com/digester-biology-top-10/