[Hatem]

Good Question Natalia.

Specifically, determining whether the Organic Loading Rate (OLR) is optimal best :

During the design stage of the biogas plant—closely linked with the Biochemical Methane Potential (BMP) test—as well as during start-up, after changes in feedstock, before scaling up, following operational interruptions, or when signs of instability appear (e.g., pH drop, VFA rise, reduced gas yield).

Regular monitoring of key indicators like biogas production, pH, alkalinity, VFA levels, and methane yield is essential.

Gradually increasing the OLR while observing for stress signals helps identify the optimal point where efficiency is maximized without compromising microbial stability.

Thanks for your question